Regulatory
sRBS Pehs

Part:BBa_K316000:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-20)


Design Notes

Designed for minimal basal transcription by altering binding site sequence and distance. The RBS was specifically designed using Vogit lab RBS calculataor[http://voigtlab.ucsf.edu/software/] to work in the context of enhanced hyperspank promoter and BBa_K316012. The promoter and RBS are coding on the negative strand to minimise read-through from upstream sequences.


Source

The part was synthesised by mwg eurofins[http://www.eurofinsdna.com/home.html] together with BBa_K316012.


Reverse strand coding Enhanced LacI-hyperspank promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


For more information about our project please visit our wiki [http://2010.igem.org/Team:Imperial_College_London].